Inspirational cases

Winner 2025
Chile
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#Environment

Young scientists transform bacteria into an ally of sustainable agriculture

How Project-Based Learning led two students to research, experiment and create a sustainable biofertilizer.

Teacher

Foto de Macarena Álvarez
Macarena Álvarez

Schools

Liceu Bicentenário de Excelência Polivalente São Nicolau

Project name

Fixaterra

STEM areas

Sciences, Technology

Other areas of knowledge

Environmental Education, Química

In the face of drought, frost and soil degradation affecting small farmers in central Chile, two Chilean students developed “Fixaterra”, a sustainable biofertilizer made from a naturally occurring bacterium that promotes plant growth and increases plants’ resilience to climate stress. What started as school research ended up becoming the country’s winning Solve for Tomorrow project in 2025.

The two girls were 14 years old and in their second year of high school (the penultimate year of compulsory schooling). The idea was born when they observed the difficulties faced by small farmers in their community due to frost, droughts and soil degradation. In a commune where about 50% of the population lives from family farming, the students wanted to find a way to protect crops from the effects of climate change. “Most farmers rely on controlled release NPK fertilizers. The problem is that these products are coated with synthetic polymers derived from petroleum, which contaminate the soil and degrade aquatic ecosystems,’ explains the mediator teacher Macarena Álvarez.

To solve this problem, they initially thought of developing genetically more resistant plants, but the teacher warned that it was necessary to turn that proposal into a viable solution for a school laboratory. “When it comes to thinking about ideas, it’s encouraging to see students dreaming of launching a satellite into space, but as teachers we have to guide them and turn that idea into something feasible,” says Álvarez.

Thus, they began research on species able to withstand extreme conditions until they found in the scientific literature the potential of Rhizobium, a bacterium that lives naturally in the soil and establishes a mutually beneficial relationship with leguminous plants. When a seed germinates, its roots release substances that attract these bacteria. Once they reach the root, they form an biological association in which the plant provides them with shelter and food, while the bacteria capture the nitrogen present in the air and transform it into a nutrient that the plant can absorb to grow, reducing the need for chemical fertilizers.

Initially, the students worked directly with roots containing the bacteria, performing small laboratory tests to observe their behavior. Then, trials began with different plant species, especially coriander, which became the main crop because it germinated quickly, was widely used by local farmers and allowed easy measurement of variables such as leaf numbers, root length and growth rate.

With the support of another teacher from the establishment specializing in chemistry, they managed to make the first capsules, manually. The result exceeded expectations: bacteria could be kept alive within small biodegradable spheres, using a source of sugar as a nutrient, until they were incorporated into the soil.

Thus was born the definitive format of “Fixaterra”, a line of biodegradable fertilizers designed to strengthen plant growth, increase their resistance against extreme weather conditions and contribute to soil regeneration, without leaving plastic waste or chemicals.

The product consists of two types of complementary microcapsules. The first type, of soft texture, contains  sucrose and the bacteria Rhizobium, a microorganism naturally present in the roots of legumes that has the ability to fix nitrogen from the air and transform it into essential nutrients for plants. The latter provide minerals such as phosphorus and potassium, fundamental for plant development. Both microcapsules are made using an alginate matrix, a biodegradable material that allows for slow and controlled release of its components into the soil.

During the tests carried out by the students, the seeds treated with “Fixaterra” germinated earlier and developed larger and more vigorous cotyledons, indicating better nutrient absorption. In addition to its environmental benefits, the creators highlight that it is a low-cost solution with potential to scale production and benefit more farmers.

Estudiantes trabajan con tubos de ensayo en laboratorio escolar de biofertilizantes

Learn by researching, mislearning, and improving

The transition between laboratory and field was one of the most delicate moments in the project, because they moved from a controlled laboratory environment to real cultivation conditions. “We couldn’t deliver a product to the farmers without evidence. They said to us: “You can make mistakes in the laboratory, we cannot make mistakes with our crops,” recalls the educator.

As a result, field trials were conducted gradually, through small-scale trials involving five farmers in the community. Beyond validating the functioning of the biofertilizer in real conditions, this stage allowed students to understand the importance of listening to users, collecting feedback and continuously improving the solution before thinking about a larger-scale application. Prior to the national finals of Solve for Tomorrow, the team had tested around 50 plants, as well as pilot experiments with five local farmers.

The entire development of “Fixaterra” lasted for about a year. Far from seeking an immediate solution, the students were perfecting the project continuously, performing new tests and adjustments at roughly three-month intervals  as proposed by the Project Based Learning methodology. Each result opened new questions and each difficulty gave rise to an improvement.

When students learn to research, they are then able to learn anything, the teacher points out.

“Students begin searching for scientific articles, reading research papers, questioning sources, and making evidence-based decisions,” she adds. Although the project had a strong scientific foundation, it still lacked one important step: to transform it into a solution that could be understood by anyone. “The mentorship from Solve for Tomorrow helped a lot. We always saw it as research. They helped us turn it into a product,” she said.

From these conversations new questions arose: How to present the solution? How to explain its operation without using too much technicality? How to make a farmer want to test it?

The students then began to develop a complete visual identity for “Fixaterra”: they designed the name, label, packaging, labeling, promotional materials and even small biodegradable envelopes with coriander seeds to be delivered during the final presentation. We cared for every detail so that it looked like a product ready to go to market. We did everything ourselves,” the teacher emphasizes.

An experience that transformed the students

The educator asserts that the biggest result of the project were not the capsules, but the transformation of the students themselves. “One of the students barely spoke before the project. One minute before he took the stage, he said to me, ‘Teacher, I forgot everything.’ I replied, ‘No, you know everything.’ After the presentation, I saw her giving interviews with an impressive confidence”, notes Álvarez. The other student also discovered new ways to lead teams, dialogue with specialists and publicly defend a scientific proposal. Weeks later, both participated in another school agronomy conference and won second place. After Solve for Tomorrow, any presentation seemed easy to them,’ she says.

For the teacher, this experience was even more special because she had a 100% female team, especially in a context where only 35% of students – worldwide – enrolled in careers in these areas in higher education are women, according to 2017 data from the United Nations Educational, Scientific and Cultural Organization (UNESCO). “Being a woman and a science teacher is looking at them and knowing that I don’t just teach them chemistry or biology, I give them keys to open doors that we were once told weren’t for us,” she recounts.

When research is part of learning

The project was also made possible by a school culture that has been promoting research for several years. The school has a consolidated STEM (Science, Technology, Engineering and Mathematics) program in which different teachers simultaneously guide projects developed by teams of students.

“I am not the only teacher who does projects. We are a team of ten teachers who support research in different areas. This year, for example, I am guiding twelve projects at the same time,” reports Álvarez. Instead of traditional classes, students work autonomously, research, make decisions, and learn how to solve real problems.” 

The recognition of “Fixaterra” in Solve for Tomorrow sparked interest from other students, families, media and farmers in the region. “Now many students want to enter the STEM program because they saw that doing science from school is possible,” she says.

Currently the students continue refining the prototype and develop new trials with 200 plants of coriander, which will allow to compare results in conditions closer to the agricultural reality and analyze with greater precision the differences between experiments carried out in laboratory and those developed directly in field. “We keep improving the project. Now we are doing a much larger study to get even more solid evidence,” she explains.

Learn more about the project in the video below:

Focus on the practice!

Take a look at the teacher’s guide to turning bacteria into an ally of sustainable agriculture

Empathize

The students Amanda Garrido and Jessika Hurtado identified that droughts, frost and soil degradation were constantly affecting small farmers in the commune of San Nicolás, in the region of Ñuble, where a significant part of the population relied on family farming. From observation of this reality and conversations with their teacher, they understood that the use of conventional fertilizers contributed to soil pollution and aquatic ecosystems. In order to respond to a specific community need, they began researching alternatives that would strengthen the crops without generating additional environmental impacts.

Define

After analyzing the problem, the team set out to develop a solution that would improve plant growth and increase their resilience against climate stress through a sustainable biofertilizer. Although they initially considered creating more genetically resistant plants, professor Macarena Álvarez guided the project toward a viable proposal for a school laboratory. The search for scientific evidence led the students to identify the potential of the bacteria Rhizobium, capable of naturally fixing nitrogen and reducing dependence on chemical fertilizers. Based on this finding, they defined the scope of the project and established the first experimental trials.

Ideate

The students devised different strategies to incorporate the bacteria into the crop and began laboratory testing with roots and various plant species. They subsequently selected cilantro as an experimental crop for its rapid germination and relevance to local farmers. During the first trials, liquid application of the bacteria did not produce the expected results, which led them to reconsider the proposal. The solution arose by observing a spherification technique used in the establishment’s Gastronomy workshop. With the support of a chemistry teacher, they adapted this procedure to encapsulate bacteria in biodegradable alginate microspheres. This is how the definitive design of “Fixaterra” was born, composed of microcapsules that incorporated Rhizobium, sucrose, phosphorus and potassium to promote a gradual release of nutrients in the soil.

Prototype

The team built the prototype through a continuous process of experimentation and improvement that lasted for about a year. They manually produced the first microcapsules and evaluated their behavior in the laboratory, verifying that bacteria remained viable within the biodegradable material. The team subsequently conducted trials with about 50 plants to measure variables such as germination, leaf and root development, and nutrient absorption. At the same time, the students developed the visual identity of the project, designing the name, packaging, labels and presentation materials, with the aim of making scientific research a solution that is understandable and close to farmers.

Test

The validation of the project was carried out progressively through real-life tests with five farmers in the community. This stage allowed comparing the performance of the biofertilizer outside the laboratory, collecting user observations and making adjustments before deploying the solution on a larger scale. The trials showed that seeds treated with “Fixaterra” germinated earlier and developed larger cotyledons, indicating better nutrient absorption. The feedback obtained during the process allowed to refine the proposal and strengthen the scientific evidence that supported its operation. The project won first place in Solve for Tomorrow Chile 2025 and motivated the students to continue expanding research through new trials with 200 plants, while consolidating skills in research, scientific communication and collaborative work.

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